Chromosome 6

1. Group composition

Participating country(ies): Canada
Head: Christoph Borchers
Members:Christoph Borchers
Christopher Overall
Leonard Foster

2. Missing proteins

Protein evidence according to NeXtProt for Chromosome 6(external link):

3. Bioinformatics protocols

4. Analytical protocols

5. Biological projects

6. Biomaterials

7. Data sets

8. Special expertise

The exact position and N-terminal chemistry of a protein chain often define the biological activity of a protein. The Chris Overall group, UBC has unique expertise in defining the N (and C-termini) of proteins by proteomics. By analysis of common and rare human tissues the Overall Lab is annotating the N-termini of proteins of Chromosome 6 and other chromosomes for the HPP. Thus we are identifying:
  • Co-translational derived mature original N-terminus
  • Post-translational cleavage derived stable cleavage chains
  • Annotation of +/- Met, site of signal peptide removal, zymogen activation
  • +/- Acetylation, other modifications
  • Alternate start sites
  • Archetypical N-terminal peptides
  • SRMs to quantify amounts of cleaved vs. uncleaved
  • Neo-epitope antibodies to biologically important protein chains directed to the N or C termini after cleavage

9. Major achievements

A review of the chromosome 6 project has been published, link(external link)
Borchers et al., 'The Human Proteome Organization Chromosome 6 Consortium: Integrating chromosome-centric and biology/disease driven strategies', Journal of Proteomics 100 (2014), 60-67.

10. The neXt50 challenge